Comprehensive Engineering of CasX Holoenzyme to Create an X-Editing Toolbox with High Activity, Specificity, & Targetability
Time: 12:00 pm
day: Day 1 Track A AM
Details:
- Comprehensive and holistic engineering to increase CasX stability, DNA-binding ability, and cleavage activity to create the X-editing platform
- XE is far more active than wild-type CasX, and edits more effectively than small Cas9 variants and other engineered small Cas12 variants
- Further modifications for PAM preferences, enabling wider range of targeting and precise specificity
