A Novel Type V CRISPR System With Potent Editing Activity in Mice and Non-Human Primates
Time: 11:45 am
day: Day 2 Track A AM
Details:
- Type V CRISPR systems: structurally and functionally distinct enzymes that utilize a short guide RNA and generate staggered cuts
- MG29-1 is a novel Type V identified from a metagenomics sample taken from a deep-sea vent and exhibits minimal sequence identity to Cas12a/cpf1
- Potent editing was achieved in both mice and NHP non-viral delivery without engineering the amino acid sequence of MG29-1.
- Optimization of the guide RNA was essential to enable in vivo potency
