A Novel Type V CRISPR System With Potent Editing Activity in Mice and Non-Human Primates

Time: 11:45 am
day: Day 2 Track A AM


  • Type V CRISPR systems: structurally and functionally distinct enzymes that utilize a short guide RNA and generate staggered cuts
  • MG29-1 is a novel Type V identified from a metagenomics sample taken from a deep-sea vent and exhibits minimal sequence identity to Cas12a/cpf1
  • Potent editing was achieved in both mice and NHP non-viral delivery without engineering the amino acid sequence of MG29-1.
  • Optimization of the guide RNA was essential to enable in vivo potency