Utilising Hybrid Guide Methods to Refine Genome Editing Precision
Time: 1:50 pm
day: Day One
Details:
- Understanding how CRISPR hybrid DNA-RNA (chRDNA) guides function to increase gene editing specificity and reduce levels of off-target editing compared to traditional all-RNA systems
- Demonstration of the activity of chRDNA guides across both the Cas9 and Cas12a CRISPR systems
- Optimization of nuclear delivery to increase editing efficiency in T cell and iPSCs
