Choosing the Right Donor DNA Format
Time: 4:00 pm
day: Day Two
Details:
- The use of double-stranded DNA (dsDNA) donors is associated with poor knock-in efficiency and increased cytotoxicity and potential insertional mutagenesis, which is driven by genomic integration of dsDNA through the HDR repair processes
- Understanding modified ssODN donors as an approach to enhance efficiency for knock-ins
- Assessing preliminary data from circular single-stranded DNA (cssDNA) and its potential to mitigate dsDNA related safety concerns
